Related Experiment Video
Updated: Aug 17, 2026

Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro
Published on: May 3, 2014
Cleavage of human MDR1 mRNA by a hammerhead ribozyme
H Kobayashi1, T Dorai, J F Holland
1Department of Neoplastic Diseases, Mount Sinai School of Medicine, New York, NY 10029.
Abstract:
We designed a hammerhead ribozyme which site-specifically cleaved the GUC sequence in codon 179 of MDR1 mRNA. The cleavage site was 6 amino acids upstream from the drug binding site and was considered sufficiently close to the essential locus for P-glycoprotein function. The ribozyme cleaved the MDR1 mRNA under physiological conditions in vitro. The cleavage was dependent on ribozyme concentration and on incubation time. Mg2+ ion was essential for the cleavage. These results show that a potentially useful tool is at hand which may inactivate MDR1 mRNA and revert the multidrug resistance phenotype.
Related Concept Videos
MicroRNAs
Nuclear Export of mRNA
Nonsense-mediated mRNA Decay
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
mRNA Stability and Gene Expression
Cis-acting Elements involved in mRNA stability
Ribozymes
Ribozymes can be...
MicroRNAs

