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CD2: a functional adhesion molecule on murine B cells, involved in interleukin-4-induced aggregation
C Elenström-Magnusson1, P Altevogt, E Severinson
1Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden.
European Journal of Immunology
|April 1, 1993
Summary
Mouse B cells express CD2, a molecule also found on T cells. Anti-CD2 antibodies trigger B cell aggregation, indicating CD2
Area of Science:
- Immunology
- Cell Biology
- Molecular Biology
Background:
- CD2 is a T cell marker, but its presence on mouse B cells was previously noted.
- The function of CD2 on B cells was not well understood.
Purpose of the Study:
- To investigate the role of CD2 in B cell adhesion.
- To determine if CD2 acts as an adhesion molecule on B cells.
Main Methods:
- Using monoclonal anti-CD2 antibody 12-15 and its fragments (F(ab')2 and Fab monomers).
- Inducing B cell aggregation in purified, resting B cells.
- Assessing the effects of temperature, metabolic energy, and divalent cations on aggregation.
- Investigating the role of CD2 in Interleukin-4-induced B cell adhesion.
Main Results:
- Anti-CD2 antibody 12-15 induced B cell homotypic adhesion.
- B cell aggregation was dose-related, temperature-dependent, and required metabolic energy and divalent cations.
- Fab monomers of anti-CD2 inhibited aggregation, suggesting CD2 is an adhesion molecule.
- Anti-CD2 antibodies inhibited Interleukin-4-induced B cell aggregation, confirming CD2's role.
Conclusions:
- CD2 functions as an adhesion molecule on mouse B cells.
- CD2 mediates both spontaneous and Interleukin-4-induced B cell homotypic adhesion.
- This finding expands the known functions of CD2 beyond T cell interactions.