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Comparison of two ribosomal DNA-based methods for differentiating members of the Anopheles gambiae complex (Diptera:
K A Taylor1, S M Paskewitz, R S Copeland
1Centers for Disease Control-Kenya, Nairobi.
Journal of Medical Entomology
|March 1, 1993
Abstract:
Two DNA-based methods, the restriction fragment length polymorphism (RFLP) and polymerase chain reaction (PCR), were used to identify mosquitoes of the Anopheles gambiae Giles complex collected in Kenya. Field-collected specimens of An. gambiae, An. arabiensis Patton, and An. merus Donity were tested. From a sample of 208 mosquitoes, 181 (87%) were identified by the RFLP method and 205 (99%) were identified by the PCR method. There was complete concordance between the two methods with regard to species identification. PCR assays were simpler, faster, and more reliable than RFLP assays.