Morphometric and neurochemical changes in rat superior cervical ganglion during growth and adulthood

We have previously found in rat superior cervical ganglion that there is a fall in neuron numbers and in cellular neuropeptide Y (NPY) immunoreactivity over the first year of life. In this study, we have used computerized image analysis to quantitate changes in neuron size, numbers and immunoreactivity for neuron-specific enolase (NSE), tyrosine hydroxylase (TH) and NPY in this ganglion between 1 and 85 weeks of age. Neuronal cytoplasmic area increased between 1 and 11 weeks and then remained stable until after 67 weeks, when there was a further increase in size of both NPY-positive and NPY-negative cells. Numbers of NPY-positive cells, but not those of NPY-negative cells, fell between 11 and 30 weeks. Both populations were stable after this time. Neuronal levels of NSE and TH remained constant, but levels of NPY fell between 58 and 67 weeks. These results indicate that changes in adult neuronal size, numbers and neurochemistry are independent phenomena. The increase in cell size and the fall in NPY after 58 weeks correspond to the time of withdrawal of some preganglionic inputs and may be responses to this event. By contrast, the decrease in NPY-positive cell numbers occurs some months before this time.