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Updated: Aug 19, 2026

Colorimetric Analysis of Alkaline Phosphatase Activity in S. aureus Biofilm
Published on: April 12, 2019
Kinetic properties of osseous plate alkaline phosphatase from diabetic rats
A A Rezende1, S O Petenusci, E C Urbinati
1Departamento de Química, F.F.C.L.R.P., Universidade de São Paulo, Ribeirão Preto, Brasil.
Abstract:
1. Increased levels of bone alkaline phosphatase activity were observed in diabetic rats. These animals exhibited impaired bone development without concomitant alterations of the sequence of cellular transformations. 2. Alkaline phosphatase activity was delayed in diabetic rats but the kinetic parameters for the hydrolysis of p-Nitrophenylphosphate (PNPP) were virtually the same observed for controls (N = 1.2 and K0.5 = 43 microM). 3. Alkaline phosphatase from diabetic rats had a better affinity (K0.5 = 38 microM) for magnesium ions than controls (K0.5 = 91 microM). 4. Zinc ions affected alkaline phosphatase activity from control and diabetic rats in the same way (K0.5 = 10 microM).

