Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Neuronal synchronization without calcium-dependent synaptic transmission in the hypothalamus

Y Bouskila1, F E Dudek

  • 1Mental Retardation Research Center, University of California, School of Medicine, Los Angeles 90024.

Proceedings of the National Academy of Sciences of the United States of America
|April 15, 1993
PubMed
Summary

Synchronous neuronal activity in the mammalian brain, particularly the hypothalamus, can occur without calcium-dependent synaptic transmission. This finding reveals alternative mechanisms for synchronizing neuronal networks.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Background suppression of electrical activity is a potential biomarker of subsequent brain injury in a rat model of neonatal hypoxia-ischemia.

Journal of neurophysiology·2022
Same author

A rodent model of human organophosphate exposure producing status epilepticus and neuropathology.

Neurotoxicology·2016
Same author

Mechanisms of Neuronal Silencing After Cortical Spreading Depression.

Cerebral cortex (New York, N.Y. : 1991)·2016
Same author

Temporal progression of evoked field potentials in neocortical slices after unilateral hypoxia-ischemia in perinatal rats: Correlation with cortical epileptogenesis.

Neuroscience·2016
Same author

Ischemic injury suppresses hypoxia-induced electrographic seizures and the background EEG in a rat model of perinatal hypoxic-ischemic encephalopathy.

Journal of neurophysiology·2015
Same author

Co-administration of subtherapeutic diazepam enhances neuroprotective effect of COX-2 inhibitor, NS-398, after lithium pilocarpine-induced status epilepticus.

Neuroscience·2014

Area of Science:

  • Neuroscience
  • Chronobiology
  • Cellular Electrophysiology

Background:

  • Neuronal synchronization is crucial for brain function, especially in the hypothalamus for regulating endocrine and autonomic systems.
  • Calcium (Ca2+)-dependent synaptic transmission is the widely accepted primary mechanism for adult mammalian brain communication.

Purpose of the Study:

  • To investigate if synchronous neuronal activity in the suprachiasmatic nucleus can occur independently of Ca2+-dependent synaptic transmission.
  • To explore alternative mechanisms for neuronal network synchronization in the mammalian hypothalamus.

Main Methods:

  • Extracellular recordings of neuronal activity in hypothalamic slices.
  • Utilizing Ca2+-free medium to block chemical synaptic transmission.
  • Whole-cell patch-clamp recordings and application of receptor antagonists (NMDA, non-NMDA, GABA-A).

Related Experiment Videos

Main Results:

  • Confirmed a circadian rhythm of synchronized activity in the suprachiasmatic nucleus.
  • Ca2+-free medium induced synchronized neuronal bursts without affecting burst synchrony with receptor antagonists.
  • Patch-clamp recordings verified the blockade of synaptic transmission in Ca2+-free conditions.

Conclusions:

  • Synchronous neuronal activity in the mammalian hypothalamus can be mediated by mechanisms independent of Ca2+-dependent synaptic transmission.
  • These alternative synchronization mechanisms may play a significant role in various synchronized neuronal networks.