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Related Experiment Videos

Tissue factor is expressed on monocytes during simulated extracorporeal circulation

J Kappelmayer1, A Bernabei, L H Edmunds

  • 1Sol Sherry Thrombosis Research Center, Temple University School of Medicine, Philadelphia, Pa. 19140.

Circulation Research
|May 1, 1993
PubMed
Summary

Simulated extracorporeal circulation stimulates monocytes to express tissue factor (TF), increasing blood clotting risk. This TF expression, particularly on recovered cells, highlights potential complications during procedures like cardiopulmonary bypass.

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Area of Science:

  • Cardiovascular Science
  • Hematology
  • Biomedical Engineering

Background:

  • Extracorporeal circulation, such as cardiopulmonary bypass, necessitates anticoagulation to prevent thromboembolic events.
  • Monocytes are key immune cells that can initiate coagulation pathways.

Purpose of the Study:

  • To investigate if monocytes express tissue factor (TF) during simulated extracorporeal circulation.
  • To quantify TF expression and procoagulant activity (PCA) on monocytes.

Main Methods:

  • Simulated extracorporeal circulation model.
  • Flow cytometry using TF-specific monoclonal antibodies.
  • Functional assay for procoagulant activity (PCA).
  • Analysis of CD11b/18 (Mac-1) expression.

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Main Results:

  • Monocyte TF expression and PCA significantly increased between 2 and 6 hours of simulated extracorporeal circulation.
  • TF levels were highest on leukocytes recovered from the oxygenator membrane.
  • CD11b/18 expression paralleled TF expression on monocytes.

Conclusions:

  • Prolonged simulated extracorporeal circulation induces TF expression on monocytes.
  • This TF expression may contribute to thrombotic risk in patients undergoing extracorporeal circulation.
  • Monocyte-derived TF and CD11b/18 may play a role in coagulation during these procedures.