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Construction of a basic genetic map for alfalfa using RFLP, RAPD, isozyme and morphological markers
G B Kiss1, G Csanádi, K Kálmán
1Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Szeged.
Molecular & General Genetics : MGG
|April 1, 1993
Summary
This study presents the first genetic map for alfalfa (Medicago sativa), detailing eight linkage groups and over 659 centimorgans. This map aids in understanding alfalfa
Area of Science:
- Plant genetics
- Genomics
- Molecular biology
Background:
- Alfalfa (Medicago sativa) is a vital forage crop with complex genetics.
- Developing a comprehensive genetic map is crucial for crop improvement and understanding its genome.
Purpose of the Study:
- To construct the first genetic map for alfalfa (Medicago sativa).
- To identify and order molecular and morphological markers across linkage groups.
- To estimate the genome size and marker density for Medicago.
Main Methods:
- Utilized a segregating population of 138 individuals from a cross between Medicago sativa ssp. quasifalcata and M. sativa ssp. coerulea.
- Employed 89 markers including RFLP, RAPD, isozyme, and morphological markers.
- Ordered markers based on linkage analysis to establish linkage groups.
Main Results:
- Established eight linkage groups representing the haploid chromosome set of Medicago.
- Unambiguously determined the order of markers spanning over 659 centimorgans.
- Calculated a haploid genome size of 1.0 x 10^9 bp, resulting in < 1500 kb per centimorgan.
Conclusions:
- The developed genetic map provides a foundational resource for alfalfa genetics and breeding.
- Despite distorted segregation, a robust genetic map was constructed, revealing insights into Medicago genome organization.
- The map's marker density offers potential for future gene mapping and marker-assisted selection in alfalfa.