Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Drosophila chromatin: an immunological study

D B Roberts, P W Andrews

    Nucleic Acids Research
    |August 8, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Researchers developed antibodies to study antigen distribution in chromatin and chromosomal proteins. Washing with saline solution removed non-specific precipitation, enabling clearer antigen analysis in Drosophila.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    ASBESTOS FIBERS MEDIATE THE UPTAKE OF DNA INTO PRIMATE CELLS IN CULTURE.

    Federation proceedings·2018
    Same author

    Acquired Genetic and Epigenetic Variation in Human Pluripotent Stem Cells.

    Advances in biochemical engineering/biotechnology·2017
    Same author

    Drosophila larval fat body surfaces : Changes in transplant compatibility during development.

    Wilhelm Roux's archives of developmental biology·2017
    Same author

    Points to consider in the development of seed stocks of pluripotent stem cells for clinical applications: International Stem Cell Banking Initiative (ISCBI).

    Regenerative medicine·2015
    Same author

    Aza-deoxycytidine induces apoptosis or differentiation via DNMT3B and targets embryonal carcinoma cells but not their differentiated derivatives.

    British journal of cancer·2014
    Same author

    Signalling of DNA damage and cytokines across cell barriers exposed to nanoparticles depends on barrier thickness.

    Nature nanotechnology·2011
    Same journal

    Correction to 'New origin firing is inhibited by APC/CCdh1 activation in S-phase after severe replication stress'.

    Nucleic acids research·2026
    Same journal

    VeloRM: disentangling pre- and post-splicing RNA modification dynamics at single-cell resolution.

    Nucleic acids research·2026
    Same journal

    Accessibility of telomeric overhangs to stabilizing small-molecule ligands.

    Nucleic acids research·2026
    Same journal

    Multivalent interactions mediate SNAIL transcription factor stimulation of the nucleosome deacetylase activity of the CoREST complex.

    Nucleic acids research·2026
    Same journal

    Genome-wide mapping of DNA G-quadruplexes in Trypanosoma brucei chromatin reveals enrichment in coding regions and transcription start sites.

    Nucleic acids research·2026
    Same journal

    Correction to 'The Gene Ontology knowledgebase in 2026'.

    Nucleic acids research·2026
    See all related articles

    Area of Science:

    • Molecular Biology
    • Immunology
    • Developmental Biology

    Background:

    • Antibodies are crucial tools for identifying and localizing specific molecules within cells.
    • Chromatin and chromosomal proteins are fundamental components of the cell nucleus, involved in DNA packaging and gene regulation.
    • Drosophila melanogaster is a widely used model organism in biological research due to its genetic tractability and conserved biological processes.

    Purpose of the Study:

    • To generate antibodies against Drosophila larval chromatin, chromosomal proteins, and cytoplasm.
    • To investigate the distribution of antigens within chromatin and chromosomal protein preparations.
    • To characterize the specificity of the generated antisera.

    Main Methods:

    • Antibody production against purified chromatin, chromosomal proteins, and larval cytoplasm from Drosophila.

    Related Experiment Videos

  • Double diffusion assays were employed to analyze antigen-antibody interactions.
  • Washing steps with a saline solution (0.06M NaCl) were used to eliminate non-specific binding.

    • Main Results:

    • Antisera against chromatin and chromosomal proteins showed precipitation with both chromatin and DNA in aqueous solutions.
    • Non-specific precipitation of chromatin and DNA by the antisera was observed.
    • Washing the immune complexes with 0.06M NaCl effectively removed this non-specific precipitation.

    Conclusions:

    • The study successfully generated antibodies for analyzing Drosophila chromatin and chromosomal proteins.
    • A method involving saline washing was established to remove non-specific antibody binding, improving assay specificity.
    • These antibodies and refined methods can be utilized for further investigations into the composition and organization of Drosophila chromatin.