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Chaperonins dependent increase of Cu,Zn superoxide dismutase production in Escherichia coli
A Battistoni1, M T Carri, C Steinkühler
1Department of Biology, University of Rome Tor Vergata, Italy.
FEBS Letters
|May 3, 1993
Abstract:
Over-expression of the chaperonins GroEL and GroES significantly suppressed the temperature-dependent pattern of expression of Cu,Zn superoxide dismutases in Escherichia coli and increased the yield of active enzyme. The results obtained indicate that chaperonins prevent degradation of metal-deficient enzyme molecules. GroEL was shown to form a complex with unfolded Cu,Zn superoxide dismutase in vitro, confirming that GroEL can interact with beta-stranded proteins.