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Related Experiment Videos

CD11b/CD18-dependent polymorphonuclear leucocyte interaction with matrix proteins in adhesion and migration

E Lundgren-Akerlund1, A M Olofsson, E Berger

  • 1La Jolla Institute for Experimental Medicine, CA.

Scandinavian Journal of Immunology
|May 1, 1993
PubMed
Summary

Monoclonal antibody 60.3 (anti-CD18) inhibits polymorphonuclear leucocyte (PMN) adhesion and migration, particularly to collagen type I. Antibody Mo1 (anti-CD11b) primarily affects PMN adhesion.

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Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Polymorphonuclear leucocytes (PMNs) play a crucial role in inflammatory responses.
  • Adhesion and migration of PMNs are critical steps in their function.
  • Matrix proteins like collagen, fibronectin, and laminin are key components of the extracellular matrix that influence PMN behavior.

Purpose of the Study:

  • To investigate the role of CD18 and CD11b molecules in PMN adhesion and migration.
  • To determine the specific matrix proteins involved in these processes.
  • To elucidate the function of monoclonal antibodies 60.3 (anti-CD18) and Mo1 (anti-CD11b).

Main Methods:

  • Human PMNs were stimulated with phorbol myristate acetate (PMA).
  • Adhesion assays were performed on plastic dishes coated with laminin (LM), fibronectin (FN), collagen type I (CI), or collagen type IV (CIV).

Related Experiment Videos

  • Migration assays were conducted using polycarbonate filters coated with matrix proteins, with LTB4 as a chemoattractant.

    • Main Results:

    • Monoclonal antibody 60.3 (anti-CD18) significantly inhibited PMN adhesion to all tested matrix proteins, with the strongest effect on collagen type I (CI).
    • Antibody Mo1 (anti-CD11b) effectively inhibited adhesion to CI but had a minor effect on adhesion to other proteins.
    • MoAb 60.3 inhibited PMN migration through protein-coated filters, especially CI, while Mo1 had no effect on migration.

    Conclusions:

    • The CD18 epitope recognized by 60.3 mediates both PMN adhesion and migration.
    • The CD11b epitope recognized by Mo1 is primarily involved in PMN adhesion.
    • CD11b/CD18 is the major receptor for PMN interaction with collagen type I, while other mechanisms may be involved in interactions with LM, FN, and CIV.