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Activity loss of glutathione synthesis enzymes associated with human subcapsular cataract
W B Rathbun1, A J Schmidt, A M Holleschau
1Ophthalmology Department, University of Minnesota, Minneapolis 55455.
Purpose:
To assess the activities of the two enzymes required for glutathione synthesis, gamma-glutamylcysteine synthetase and glutathione synthetase, in various forms of human cataracts.
Methods:
The Cooperative Cataract Research Group cataract classification method and standard enzyme assay procedures were used.
Results:
An inverse relationship was shown between residual activity of each of the glutathione synthesis enzymes and degree of subcapsular cataract. A weaker inverse relationship existed between glutathione synthetase activity and supranuclear and nuclear cataracts. No other parameters yielded comparable correlations with the activity of either enzyme.
Conclusions:
Activity loss of the glutathione synthesis enzymes is associated with human subcapsular cataract formation.