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Related Experiment Videos

Chimeric human and mouse spheroids

H Walt1, P Emmerich

  • 1Department of Gynecology and Obstetrics, University Hospital, Zürich, Switzerland.

Virchows Archiv. B, Cell Pathology Including Molecular Pathology
|January 1, 1993
PubMed
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Researchers studied human teratocarcinoma cells in mice, finding they formed structures with both human and mouse cells. These bispecies formations, termed chimeric spheroids, are distinct from embryoid bodies (EBs).

Area of Science:

  • Developmental biology
  • Cancer research
  • Xenotransplantation models

Background:

  • Embryoid bodies (EBs) are crucial in developmental biology research.
  • Teratocarcinoma cells can form complex structures in vivo.
  • Distinguishing between similar biological structures is essential for accurate research.

Purpose of the Study:

  • To investigate the nature of xenografted human teratocarcinoma cells forming EB-like structures in mice.
  • To differentiate these structures from true embryoid bodies (EBs).
  • To propose a new classification for these bispecies formations.

Main Methods:

  • Intraperitoneal injection of xenografted human teratocarcinoma cells into nude mice.
  • In situ hybridization using species-specific DNA probes (human and mouse).

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  • Analysis of paraffin-embedded sections to localize cell nuclei.

    • Main Results:

    • EB-like structures were observed in vivo.
    • In situ hybridization identified two distinct cell populations: human tumor cells and mouse cells.
    • Human tumor cells were located centrally, surrounded by an outer coat of mouse cells.

    Conclusions:

    • The investigated structures are of bispecies origin, containing both human and mouse cells.
    • These structures do not meet the definition of embryoid bodies (EBs).
    • The term "chimeric spheroids" is proposed for clear distinction from EBs and other spheroids.