[The analysis of human DNA fingerprints by the synthetic oligonucleotide probe]

In order to test the practical applicability of oligonucleotide fingerprinting we have investigated 50 unrelated individuals, 11 members in two families and one pair of twin using the probe (CAC)5/(GTG)5. Except the monozygotic twins highly variable patterns were demonstrated, even in a family. The probability of chance association between random individuals was calculated as 3.8 x 10(-10). It seems reasonable to conclude that (CAC)5/(GTG)5 fingerprints are completely individual-specific. The results of the pedigree study showed that the inheritance of alleles was in keeping with the Mendelian Law. This method is not so costly and labor-intensive as the conventional Southern blotting. It increases the speed and lessen the complexity of RFLPs study.