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Related Experiment Videos

Complex duplications in maize lines

K S Hong1, T E Richter, J L Bennetzen

  • 1Department of Plant Pathology, Kansas State University, Manhattan 66506-5502.

Molecular & General Genetics : MGG
|May 1, 1993
PubMed
Summary
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Maize disease resistance gene Rp1

Area of Science:

  • Plant genetics
  • Maize molecular biology
  • Genomics

Background:

  • Rp1 is a disease resistance complex and a key marker on maize chromosome 10.
  • Understanding the structure of RFLP loci near Rp1 is crucial for maize genetics.

Purpose of the Study:

  • To investigate the structural complexity of RFLP loci located near the Rp1 gene in maize.
  • To determine if RFLP loci exhibit similar complex structures to the Rp1 gene.

Main Methods:

  • Analysis of restriction fragment length polymorphisms (RFLPs) in maize.
  • Mapping RFLP loci relative to the Rp1 gene.
  • Examining RFLP copy number variation and meiotic recombination.

Main Results:

  • Two RFLP loci distal to Rp1 were single-copy and cosegregated perfectly.

Related Experiment Videos

  • Two RFLP loci proximal to Rp1 exhibited complex structures with variable copy numbers.
  • One proximal locus showed meiotic recombination leading to copy number changes.

    • Conclusions:

    • Genomic regions near Rp1 may commonly harbor variable repeated sequences.
    • The complex structures of these RFLP loci offer insights into the evolution and organization of disease resistance genes like Rp1.