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Smoking, CD45R0+ (memory), and CD45RA+ (naive) CD4+ T cells
M Chavance1, J Y Perrot, I Annesi
1INSERM U169, Villejuif, Paris, France.
The American Review of Respiratory Disease
|July 1, 1993
Summary
Smoking significantly increases T-cell subsets, including memory (CD45R0+) and naive (CD45RA+) CD4+ lymphocytes, in middle-aged men. Both cell counts and percentages were affected, suggesting equivalent impacts on these crucial immune cell populations.
Area of Science:
- Immunology
- Public Health
Background:
- Smoking is a major global health concern.
- Its impact on immune cell populations, particularly T-cell subsets, requires further elucidation.
Purpose of the Study:
- To investigate the relationship between smoking and T-cell subsets in middle-aged men.
- To specifically assess the effects on CD45R0+ (memory) and CD45RA+ (naive) CD4+ lymphocytes.
Main Methods:
- Analysis of 311 middle-aged men from the Paris area.
- Determination of lymphocyte phenotypes (CD4+, CD8+, CD4+CD45R0+, CD4+CD45RA+) using double immunofluorescence labeling.
- Correlation of cell counts and percentages with tobacco consumption.
Main Results:
- Significant increases in total CD4+CD45R0+ and CD4+CD45RA+ lymphocyte counts were observed with increased smoking.
- Percentages of CD4+CD45R0+ and CD4+ lymphocytes significantly increased with tobacco consumption.
- The increase in CD4+CD45RA+ percentage was not statistically significant but mirrored the CD4+CD45R0+ trend, indicating equivalent effects.
Conclusions:
- Smoking positively correlates with increased counts and percentages of both memory and naive CD4+ T-lymphocytes.
- The study suggests that smoking affects these T-cell subsets similarly, impacting overall T-cell homeostasis.