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Physical mapping of inhibin beta-A in domestic cattle
H L Neibergs1, D S Gallagher, M Georges
1Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station 77843.
Abstract:
The gene for the beta-A subunit of inhibin (INHBA) was assigned to bovine syntenic group U13 by bovine x rodent hybrid somatic cells and the polymerase chain reaction (PCR). A 482-bp PCR fragment was used to clone a 37-kb cosmid. This cosmid was assigned to bovine Chromosome (Chr) 4 (BTA 4) by fluorescence in situ hybridization (FISH). This is the first assignment of a U13 marker to a bovine chromosome. A restriction fragment length polymorphism (RFLP) was detected with PstI within the INHBA cosmid.