Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA analysis demonstrates that mycococcus forms are not mycobacteria

D de Wit1, D A Mitchison

  • 1Department of Bacteriology, Royal Postgraduate Medical School, Hammersmith Hospital, London.

Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease
|April 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intralipid administration in case of a severe venlafaxine overdose in a patient with previous gastric bypass surgery.

Toxicology reports·2022
Same author

Everolimus pharmacokinetics and its exposure-toxicity relationship in patients with thyroid cancer.

Cancer chemotherapy and pharmacology·2016
Same author

Correction for Spray Dried Aerosol Particles of Pyrazinoic Acid Salts for Tuberculosis Therapy.

Molecular pharmaceutics·2015
Same author

Beneficial Effects of the mTOR Inhibitor Everolimus in Patients with Advanced Medullary Thyroid Carcinoma: Subgroup Results of a Phase II Trial.

International journal of endocrinology·2015
Same author

Midazolam as a phenotyping probe to predict sunitinib exposure in patients with cancer.

Cancer chemotherapy and pharmacology·2013
Same author

Contradictory results with high-dosage rifamycin in mice and humans.

Antimicrobial agents and chemotherapy·2013

This study investigated the mycococcus form of Mycobacterium bovis BCG and M. phlei, finding it is not derived from mycobacteria. Molecular and biochemical analyses revealed these variant forms are environmental contaminants, not true mycobacterial forms.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Mycobacterial Research

Background:

  • Previous studies suggested mycobacteria might exist in forms beyond the acid-fast bacillus.
  • Debate has persisted regarding the origin and nature of these proposed variant forms.
  • The mycococcus form, historically linked to mycobacteria, requires definitive characterization.

Purpose of the Study:

  • To conclusively determine if the mycococcus form is a variant of Mycobacterium bovis BCG and M. phlei.
  • To differentiate between true mycobacterial variants and potential contaminants.
  • To apply modern molecular techniques to resolve long-standing questions in mycobacterial morphology.

Main Methods:

  • Analysis of stored mycococcus cultures of Mycobacterium bovis BCG and M. phlei.

Related Experiment Videos

  • Comparison of DNA restriction fragment patterns between variant forms and parent mycobacteria.
  • DNA hybridization assays to assess genetic relatedness.
  • Biochemical characterization of the variant forms.

    • Main Results:

    • Restriction fragment patterns of mycococcus DNA were dissimilar to parent mycobacteria.
    • Chromosomal DNA from parent mycobacteria did not hybridize with the DNA of the mycococcus forms.
    • Biochemical studies indicated the mycococcus forms are environmental contaminants.

    Conclusions:

    • The mycococcus form is definitively not derived from Mycobacterium bovis BCG or M. phlei.
    • This study resolves the debate concerning the mycococcus as a mycobacterial variant.
    • Future research into potential mycobacterial variants should rigorously employ molecular technologies.