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Cellular retinol-binding protein

M M Bashor, F Chytil

    Biochimica Et Biophysica Acta
    |November 10, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Researchers identified a specific protein that binds retinol in various rat tissues. This cellular retinol-binding protein differs from serum retinol-binding protein and is present from early fetal development.

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    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Cell Biology

    Background:

    • Retinol (Vitamin A) is essential for numerous physiological processes.
    • Specific binding proteins facilitate retinol transport and cellular uptake.
    • Understanding these proteins is crucial for comprehending retinoid metabolism and function.

    Purpose of the Study:

    • To detect and characterize a novel protein that binds retinol in rat tissues.
    • To determine the specificity and distribution of this cellular retinol-binding protein.
    • To differentiate this protein from known serum retinol-binding proteins.

    Main Methods:

    • Sucrose gradient centrifugation and gel filtration were used to isolate and analyze the protein.
    • Competition studies with various retinoids were performed to assess binding specificity.

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  • Tissue cytosols from different adult and fetal rats, as well as tumor cell lines, were analyzed.

    • Main Results:

    • A high-affinity, specific retinol-binding protein (approximately 16,000 MW, 2 S sedimentation) was identified in rat tissue cytosols.
    • The protein exclusively binds all-trans-retinol, not retinal or retinoic acid, and binding is reversible.
    • This cellular retinol-binding protein is present in multiple tissues (liver, lung, brain, etc.) and in fetuses from day 12, but absent in certain tumors and muscle tissues.
    • It differs in size and immunological properties from serum retinol-binding protein.

    Conclusions:

    • A distinct cellular retinol-binding protein exists in rat tissues with specific ligand-binding properties.
    • This protein plays a role in retinoid transport and metabolism throughout development and in various organs.
    • The cellular retinol-binding protein is distinguishable from serum retinol-binding protein and is not found in the tested tumor cell lines.