N-ethylmaleimide inhibition of silver-enhanced sodium transport across toad (Bufo macinus) skin

1. Silver stimulated short-circuit current and transepithelial potential difference. 2. N-Ethylmaleimide inhibited the silver-induced short-circuit current. 3. There was a biphasic inhibition of silver-induced short-circuit current by N-ethylmaleimide. 4. There is a specific, maximal number of sulfhydryl groups associated with active sodium absorption. 5. Stimulation of active sodium transport by antidiuretic hormone was blunted by the presence of silver. 6. The silver-induced short-circuit current is carried by a net active sodium transfer from the outside to the inside bathing solution.