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Electrolyte distribution in toad sciatic nerve and spinal cord

C A Astrada, E Haggi, E Hliba

    Brain Research
    |November 14, 1975
    PubMed
    Summary

    This study investigated sodium (Na+) and potassium (K+) distribution in toad tissues. Findings reveal specific electrolyte concentrations in muscle and spinal cord, with unique challenges in sciatic nerve analysis due to its sheath.

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    Area of Science:

    • Physiology
    • Neuroscience
    • Comparative Biology

    Background:

    • Understanding electrolyte distribution is crucial for cellular function.
    • Previous studies have characterized ion concentrations in various tissues, but detailed analysis in amphibian nervous tissue and muscle is less common.

    Purpose of the Study:

    • To quantify the distribution of sodium (Na+) and potassium (K+) ions and water in the spinal cord, sciatic nerve, and sartorius muscle of the toad, Bufo arenarum.
    • To investigate the influence of the sciatic nerve's connective sheath on electrolyte distribution analysis.
    • To explore the localization of Na+ within nervous tissues using histochemical techniques.

    Main Methods:

    • Electrolyte assays were performed on tissue samples.
    • Sodium washout curves were utilized to assess ion dynamics.

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  • Electron microscopy was employed to determine the contribution of the sciatic nerve sheath.
  • Pyroantimoniate histochemical staining was used for Na+ localization.

    • Main Results:

    • Sartorius muscle: 18.9% extracellular water, intracellular Na+ 18.4 mEq/kg, intracellular K+ 147.9 mEq/kg.
    • Spinal cord: 17.2% extracellular water, intracellular Na+ 40.6 mEq/kg, intracellular K+ 134.5 mEq/kg.
    • Sciatic nerve sheath constituted 22.3% of nerve mass, influencing ion distribution calculations; excess Na+ and water were noted in the nerve.
    • Pyroantimoniate staining indicated significant Na+ precipitate within myelin lamellae of both spinal cord and sciatic nerve.

    Conclusions:

    • Electrolyte and water distribution varies significantly across different toad tissues.
    • The sciatic nerve sheath presents a unique challenge for accurate electrolyte distribution assessment.
    • Myelin lamellae in nervous tissue may play a role in sequestering sodium ions.