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Endogenous factor activating Na,K-ATPase induced by blockade of adrenoceptors
B N Manukhin1, P A Erokhov, E V Volina
1Institute of Developmental Biology, Russian Academy of Sciences, Moscow.
Biochemical Pharmacology
|July 6, 1993
Abstract:
It was established that the regulatory factor released from the effector cells under the blockade of postsynaptic alpha- and beta-adrenoceptors by phentolamine and propranolol (0.5-1.0 microM) and activating noradrenergic uptake and synthesis increased the activity of Na,K-ATPase of isolated rat organs and partially purified Na,K-ATPase isolated from the rat brain. The regulatory factor is a protein with molecular mass 25-100 kDa, adsorbed on phenylsepharose CL-4B and eluated with 30 mM KCl. It consists of two active components with pI 5.1 and 5.9 which in admixture (1:1) activate Na,K-ATPase by 60%.