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Hepatic oxygen consumption and cytochrome P450 activity in experimental faecal peritonitis
I Rasmussen1, A Rane, U Haglund
1Department of Surgery, University Hospital, Uppsala, Sweden.
Objective:
To study hepatic oxygen consumption and cytochrome P450 activity in pigs with septic shock induced by faecal peritonitis.
Design:
Controlled experimental study.
Animals:
12 pigs weighing 19-27 kg.
Intervention:
The animals were divided into a control group (n = 6) and a peritonitis group (n = 6). Peritonitis was induced by intraperitoneal instillation of a standard amount of autologous faeces. The animals were then observed for 300 minutes. Liver biopsy specimens were taken at 0 and 300 minutes.
Main Outcome Measures:
Hepatic oxygen delivery (DO2) and consumption (VO2). Cytochrome P450 activity was studied by measuring O- and N-demethylation of codeine at 0 and 300 minutes.
Results:
Hepatic DO2 was reduced, whereas VO2 was increased during sepsis. There were no significant changes in the N- and O-demethylation of codeine.
Conclusions:
Hepatic VO2 did increase during sepsis, possibly because of the increased metabolic demand. Cytochrome P450 activity was unaffected by the septic challenge.