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Allergic Reactions

Allergic Reactions

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Related Experiment Video

Updated: Jan 19, 2026

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Published on: June 29, 2021

Isolation and characterization of cal allergens

C R Stokes, M W Turner

Clinical Allergy

|September 1, 1975

Summary

Researchers identified two major cat allergens from skin extracts. These allergens, with distinct isoelectric points and molecular weights, were used in a RAST procedure to differentiate between individuals sensitive and non-sensitive to cats.

Area of Science:

Allergy and Immunology
Biochemistry
Protein Chemistry

Background:

Cat allergy is a common hypersensitivity reaction.
Identifying specific allergens is crucial for accurate diagnosis and immunotherapy.

Purpose of the Study:

To isolate and characterize the major allergenic components from cat skin extracts.
To develop a diagnostic tool for distinguishing between cat-allergic and non-allergic individuals.

Main Methods:

Fractionation of cat skin extract using Sephadex G200 and isoelectric focusing.
Immunoelectrophoresis and molecular weight determination.
Radioallergosorbent test (RAST) using isolated allergens.

Main Results:

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Last Updated: Jan 19, 2026

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Published on: June 29, 2021

Removal and Replacement of Endogenous Ligands from Lipid-Bound Proteins and Allergens

Removal and Replacement of Endogenous Ligands from Lipid-Bound Proteins and Allergens

Published on: February 24, 2021

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE

Published on: April 21, 2019

Two distinct allergenic fractions were identified with isoelectric points of 4.1 and 4.35.

The allergen with pI 4.35 was identified as a serum albumin-like protein (69,000 daltons).

The allergen with pI 4.1 migrated in the alpha2-region (55,000 daltons) and was analyzed for composition.

Conclusions:

Two major cat allergens were successfully isolated and characterized.
A RAST procedure utilizing these allergens effectively differentiated between cat-sensitive and non-sensitive individuals.