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Coltiviruses isolated from mosquitoes collected in Indonesia
S E Brown1, B M Gorman, R B Tesh
1Department of Entomology, College of Agricultural Sciences, Colorado State University, Fort Collins 80523.
Virology
|September 1, 1993
Summary
New Coltivirus genotypes were discovered in Indonesian mosquitoes. These viruses, distinct from Colorado tick fever virus, show unique RNA profiles and genetic relatedness, indicating significant viral diversity.
Area of Science:
- Virology
- Molecular Biology
- Arbovirology
Background:
- Mosquitoes are vectors for various viruses, including Coltiviruses.
- Coltiviruses possess segmented double-stranded RNA genomes.
- Colorado tick fever (CTF) virus is the type species of the Coltivirus genus.
Purpose of the Study:
- To characterize novel Coltivirus isolates from Indonesian mosquitoes.
- To determine the genetic relatedness of these isolates to each other and to known Coltiviruses.
- To identify potential new genotypes within the Coltivirus genus.
Main Methods:
- Isolation of viruses from mosquito pools.
- Analysis of viral genome segment electrophoretic patterns using PAGE.
- Assessment of genetic relatedness through RNA-RNA hybridization.
Main Results:
- Eleven Coltivirus isolates from Indonesia displayed distinct PAGE profiles, differing from CTF virus.
- Ten isolates showed a 6-6 segment profile with variations, while one had a unique 6-5-1 profile.
- RNA-RNA hybridization revealed limited cross-hybridization among Indonesian isolates and no cross-hybridization with CTF virus, indicating distinct genotypes.
Conclusions:
- The Indonesian Coltivirus isolates represent at least two distinct genotypes.
- These findings expand the known genetic diversity within the Coltivirus genus.
- Further research is needed to fully understand the characteristics and potential impact of these novel Coltiviruses.