A comparison of the yields of translocations and dicentrics measured using fluorescence in situ hybridization
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FISH for Pre-implantation Genetic Diagnosis
Published on: February 23, 2011
1University of California, Lawrence Livermore National Laboratory, Livermore, CA 94551.
International Journal of Radiation Biology
|August 1, 1993
Abstract:
Chromosome aberration analysis using fluorescence in situ hybridization (FISH) methods without centromere-specific markers results in the misscoring of a substantial fraction of the dicentrics as translocations; that is, too many translocations and too few dicentrics are scored. Such misscoring has led to considerable confusion in the rapidly emerging literature on FISH-based cytogenetics. Here, we demonstrate that the problem is fully resolved when centromeric probes are used.

