New aspects of dopaminergic interplexiform cell organization in the goldfish retina

Dopaminergic interplexiform cells (DA-IPCs) in the goldfish retina have been reexamined by light and electron microscopic immunocytochemistry with antisera against dopamine (DA) or tyrosine hydroxylase (TH). Successful immunostaining with a specific anti-DA antiserum offers further direct support for DA-IPCs. Anti-DA immunocytochemistry in combination with [3H]-DA autoradiography shows 92% colocalization of the two markers, indicating that [3H]-DA autoradiography is a reliable technique for identification of DA-IPCs. Incubations with anti-TH antiserum show that immunoreactive DA-IPCs have a homogeneous distribution, with an average frequency of 71 +/- 8 cells/mm2 in retinas of 14-15 cm long goldfish. Their arrangement is distinctly nonrandom. Electron microscopy of TH-immunoreactive cell processes confirms that horizontal cell axons synapse onto DA-IPCs and adds the following junctional arrangements to the circuit diagram of the DA-IPC: 1) adjacent serial synapses between DA-IPCs, external horizontal cells, and putative glycinergic interplexiform cells, 2) junctional appositions between DA-IPCs and photoreceptor cells, 3) junctional appositions between neighbouring DA-IPCs, and 4) the "gap junctional complex," typically consisting of a DA-IPC process juxtaposed with a gap junction between horizontal cell axons. The gap junction is flanked by clusters of small, round vesicles and groups of electron-dense structures resembling intermediate filaments. These morphological results support the functional involvement of DA-IPCs in adaptive retinomotor movements and in horizontal cell gap junction modulation and/or dynamics. They also suggest particular interaction between the dopaminergic and the glycinergic IPC system in the outer plexiform layer of goldfish retina.