Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Inositol trisphosphate receptor and Ca2+ signalling

K Mikoshiba1, T Furuichi, A Miyawaki

  • 1Department of Molecular Neurobiology, University of Tokyo, Japan.

Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
|June 29, 1993
PubMed
Summary

Inositol 1,4,5-trisphosphate receptor (InsP3-R) functions as a calcium (Ca2+) release channel. This study identifies InsP3-R as the P400 protein and details its structure, function, and regulation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of the school lunch program on overweight and obesity among junior high school students: a nationwide study in Japan.

Journal of public health (Oxford, England)·2018
Same author

3D modeling of the electron energy distribution function in negative hydrogen ion sources.

The Review of scientific instruments·2010
Same author

A case of small cell carcinoma in the buccal region.

International journal of oral and maxillofacial surgery·2009
Same author

Effect of energy relaxation of H(0) atoms at the wall on the production profile of H(-) ions in large negative ion sources.

The Review of scientific instruments·2008
Same author

Analysis of electron energy distribution of an arc-discharge H(-) ion source with Monte Carlo simulation.

The Review of scientific instruments·2008
Same author

Dynamic behavior of individual cells in developing organotypic brain slices revealed by the photoconvertable protein Kaede.

Experimental neurology·2006

Area of Science:

  • Cellular Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Inositol 1,4,5-trisphosphate (InsP3) is a crucial second messenger mediating intracellular calcium (Ca2+) release.
  • The InsP3 receptor (InsP3-R) is the primary protein responsible for this calcium release.
  • Understanding InsP3-R's structure and function is vital for deciphering calcium signaling pathways.

Purpose of the Study:

  • To identify and characterize the InsP3 receptor (InsP3-R).
  • To elucidate the role of InsP3-R in calcium release from intracellular stores.
  • To investigate the structural and regulatory properties of InsP3-R.

Main Methods:

  • Purification and cDNA cloning of the InsP3 receptor.
  • Generation of a cell transfectant expressing InsP3-R.

Related Experiment Videos

  • Biochemical assays including lipid bilayer incorporation and immunogold labeling.
  • Cross-linking experiments and sequence conservation analysis.

    • Main Results:

    • InsP3-R is identical to the P400 protein, enriched in cerebellar Purkinje cells.
    • Expressed InsP3-R binds InsP3 with high affinity and mediates InsP3-induced Ca2+ release.
    • InsP3-R forms a homotetramer, localized to the endoplasmic reticulum.
    • Conserved N-terminal sequences are critical for InsP3 binding; novel subtypes exist.

    Conclusions:

    • InsP3-R functions as a ligand-gated Ca2+ release channel.
    • The P400 protein is the InsP3 receptor, a key component of calcium signaling.
    • Structural conservation and alternative splicing contribute to InsP3-R diversity and function.