Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Analysis of one-dimensional electrophoregrams

A Trubuil1

  • 1INRA, Laboratoire de Biométrie, Jouy en Josas, France.

Computer Applications in the Biosciences : CABIOS
|August 1, 1993
PubMed
Summary

This study presents novel software for analyzing one-dimensional electrophoresis gels from digitized images. It corrects distortions and aids in genetic family studies, improving accuracy in molecular weight estimation and genotype determination.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

3-D aggregated object detection and labeling from multivariate confocal microscopy images: a model validation approach.

IEEE transactions on systems, man, and cybernetics. Part B, Cybernetics : a publication of the IEEE Systems, Man, and Cybernetics Society·2008
Same author

Human intestinal circadian clock: expression of clock genes in colonocytes lining the crypt.

Chronobiology international·2006
Same author

[Lupus erythematosus profundus].

Annales de dermatologie et de venereologie·1989
Same author

Electrical excitability of artificial enzyme membranes. IV. Theoretical approach of the membrane potential of synthetic proteinic films.

Biophysical chemistry·1988

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • One-dimensional electrophoresis is crucial for molecular weight estimation, genotype determination, and DNA sequencing.
  • Existing software offers semi-automatic to automatic gel analysis, focusing on lanes, bands, and optical density profiles.

Purpose of the Study:

  • To develop and present an approach for one-dimensional gel analysis using digitized images.
  • To address global distortion correction caused by inhomogeneous electric fields.
  • To enhance gel interpretation, particularly for genetic studies of families.

Main Methods:

  • Utilizing digitized images for one-dimensional gel analysis.
  • Implementing algorithms for the detection of lanes and bands.
  • Developing methods for quantitative analysis and fragment size calculation.
  • Focusing on correcting global distortions and improving gel interpretation.

Main Results:

  • A software package is presented that includes standard gel analysis facilities.
  • Particular attention is paid to correcting distortions and facilitating interpretation.
  • The approach is tailored for genetic studies of families, enhancing data reliability.

Conclusions:

  • The developed software provides a robust solution for one-dimensional gel analysis.
  • It offers improved accuracy in molecular weight estimation and genotype determination.
  • The focus on distortion correction and interpretation makes it valuable for genetic research.

Related Experiment Videos