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Nitric oxide regulates luteinizing hormone-releasing hormone secretion
M Moretto1, F J López, A Negro-Vilar
1Reproductive Neuroendocrinology Section, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709.
Endocrinology
|November 1, 1993
Summary
Nitric oxide (NO) stimulates luteinizing hormone-releasing hormone (LHRH) secretion by activating cGMP signaling pathways. This finding highlights NO's role in regulating reproductive functions.
Area of Science:
- Neuroendocrinology
- Reproductive Biology
Background:
- Nitric oxide (NO) is an emerging neuromodulator.
- Luteinizing hormone-releasing hormone (LHRH) regulates reproductive functions.
Purpose of the Study:
- To investigate the role of NO in LHRH secretion.
- To elucidate the signaling pathway involved in NO-mediated LHRH release.
Main Methods:
- In vitro studies using hypothalamic explants and GT1 neuronal cell lines.
- Administration of NO donor (sodium nitroprusside) and cGMP analog (8-Br-cGMP).
- Measurement of intracellular and extracellular cGMP levels.
- Inhibition studies using Rp-8-Br-cGMPS.
Main Results:
- Sodium nitroprusside dose-dependently stimulated LHRH release.
- SNP treatment increased intracellular and extracellular cGMP levels.
- 8-Br-cGMP mimicked NO's effect, enhancing LHRH release.
- Rp-8-Br-cGMPS blocked NO-induced LHRH secretion, implicating cGMP-dependent protein kinase.
Conclusions:
- NO stimulates LHRH secretion via activation of guanylate cyclase and the cGMP pathway.
- NO acts as a neuroactive agent controlling LHRH secretion.
- These findings suggest a role for NO in the regulation of reproductive functions.