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Transglutaminase covalently incorporates amines into human immunodeficiency virus envelope glycoprotein gp120 in
L Mariniello1, C Esposito, V Gentile
1Department of Biochemistry and Biophysics, University of Naples, Italy.
International Journal of Peptide and Protein Research
|August 1, 1993
Abstract:
Human immunodeficiency virus envelope glycoprotein gp120, but not its precursor gp160, covalently incorporates both spermidine and glycine ethyl ester in the presence of Ca2+ and transglutaminase purified from guinea pig liver. The examined ability to act as enzyme substrate of various glutamine-containing gp120 fragments, including the principal neutralizing determinant, the CD4 binding domain, and the sequence 254-274, suggested to be involved in post-binding events and in virus entry in the host cell, indicated the glutamine-265 as possible reactive acyl donor site of the protein.