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Related Experiment Videos

Characterization of monocyte-activating tumour cell membrane structures

U Westenfelder1, B Schraven, D N Männel

  • 1Division for Immunochemistry, German Cancer Research Center, Heidelberg.

Scandinavian Journal of Immunology
|October 1, 1993
PubMed
Summary

Tumor cells stimulate monocytes via plasma membrane components, specifically a 45 kDa glycoprotein. Altered glycosylation of these tumor cell surface molecules may drive this monocyte activation and tumor necrosis factor induction.

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Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Tumor cells are known to activate monocytes/macrophages.
  • Tumor-cell membranes confer this stimulatory effect.

Purpose of the Study:

  • To analyze structures responsible for tumor cell-specific tumor necrosis factor (TNF) induction.
  • To identify the molecular components on tumor cell membranes that activate monocytes.

Main Methods:

  • Monocytes from healthy donors were cultured with plasma membrane preparations from Jurkat and K562 cells.
  • Characterization of the TNF-inducing factor's properties, including molecular weight and glycosylation.

Main Results:

  • A 45 kDa plasma membrane component from both Jurkat and K562 cells stimulated monocytes.

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  • The TNF-inducing factor is a glycoprotein, with the carbohydrate moiety being crucial for stimulation.
  • CD2 was identified as an activating structure on Jurkat cells; the structure on K562 cells remains unknown.

    • Conclusions:

    • Carbohydrate structures are critical for monocyte activation by tumor cell membranes.
    • Altered glycosylation of tumor cell surface molecules may be responsible for tumor cell-induced monocyte stimulation and TNF production.