Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

One-ended insertion of IS911

P Polard1, L Seroude, O Fayet

  • 1UPR 9007 du Centre National de la Recherche Scientifique, Toulouse, France.

Journal of Bacteriology
|February 1, 1994
PubMed
Summary

The insertion sequence IS911 mediates a nonreplicative integration reaction involving its right-end inverted repeat (IRR) and flanking DNA. This process suggests transposase recognition of IRR in cis, influencing integration efficiency.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparative Effectiveness of Behavioral Interventions to Prevent or Delay Dementia: One-Year Partner Outcomes.

The journal of prevention of Alzheimer's disease·2020
Same author

Characteristics of Minor Ions and Electrons in Flux Transfer Events Observed by the Magnetospheric Multiscale Mission.

Journal of geophysical research. Space physics·2020
Same author

World Small Animal Veterinary Association Global Dental Guidelines.

The Journal of small animal practice·2020
Same author

World Small Animal Veterinary Association Global Dental Guidelines.

The Journal of small animal practice·2020
Same author

Recombination of the Phase-Variable <i>spnIII</i> Locus Is Independent of All Known Pneumococcal Site-Specific Recombinases.

Journal of bacteriology·2019
Same author

Surveillance of traffic incident management-related occupational fatalities in Kentucky, 2005-2016.

Traffic injury prevention·2018

Area of Science:

  • Molecular Biology
  • Genetics
  • Microbiology

Background:

  • Insertion sequences (IS) are mobile genetic elements that can transpose within genomes.
  • IS911 is a non-replicative transposon, meaning it does not duplicate itself during transposition.
  • Understanding the mechanism of IS911 integration is crucial for comprehending its role in genome dynamics.

Purpose of the Study:

  • To analyze the nonreplicative integration reaction mediated by the insertion sequence IS911.
  • To investigate the roles of the right-end inverted repeat (IRR) and flanking DNA sequences in IS911 integration.
  • To explore the potential influence of transposase proximity on IRR recognition and integration efficiency.

Main Methods:

  • Site-directed mutagenesis was used to alter sequences in the flanking vector DNA.
  • Integration reactions were analyzed to assess the impact of sequence modifications.
  • Comparative analysis of integration efficiency under different conditions was performed.

Main Results:

  • The integration reaction involves the IS911 right-end inverted repeat (IRR) and flanking vector DNA sequences.
  • Flanking sequences function as a surrogate IS911 end, essential for efficient integration.
  • Eliminating sequence similarities between flanking DNA and IRR significantly reduces integration.
  • Data suggest that transposase gene proximity to IRR enhances its activity, promoting cis recognition.

Conclusions:

  • The IS911 integration mechanism relies on the interaction between the IRR and flanking DNA.
  • Flanking DNA acts as a crucial recognition site, mimicking an IS911 end.
  • Preferential transposase recognition of IRR in cis, potentially due to proximity, is a key factor in the integration process.

Related Experiment Videos