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H-ras gene mutations in salivary gland pleomorphic adenomas
J Milasin1, N Pujić, N Dedović
1Institute of Biology and Human Genetics, School of Stomatology, Belgrade, Yugoslavia.
Abstract:
The DNA from 17 specimens of pleomorphic adenoma of the salivary glands was screened for the presence of ras gene mutations, which are known to be involved in the pathogenesis of various human neoplasias. By a sensitive method of hybridization with synthetic oligonucleotide probes on in vitro amplified tumor DNA, point mutations, mainly in codon 12 of the H-ras gene, were detected in six tumor specimens (35%). This high incidence of mutated ras genes suggests that their alteration may play a role in the pathogenesis of pleomorphic adenomas.
Insights
Researchers investigated ras gene mutations in pleomorphic adenoma, a salivary gland tumor. They found mutations in the H-ras gene in 35% of specimens, suggesting a role in tumor development.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Pleomorphic adenomas are the most common salivary gland tumors.
- Ras genes are frequently implicated in human neoplasia pathogenesis.
Purpose of the Study:
- To investigate the presence and potential role of ras gene mutations in pleomorphic adenomas of the salivary glands.
Main Methods:
- DNA was extracted from 17 pleomorphic adenoma specimens.
- Tumor DNA was amplified in vitro.
- Hybridization with synthetic oligonucleotide probes was used to detect point mutations in the ras gene, specifically focusing on codon 12 of the H-ras gene.
Main Results:
- Ras gene mutations were detected in 6 out of 17 tumor specimens (35%).
- The majority of detected mutations were point mutations in codon 12 of the H-ras gene.
Conclusions:
- The high incidence of ras gene mutations in pleomorphic adenomas suggests their significant involvement in the pathogenesis of these salivary gland tumors.
- Targeting ras gene alterations may offer potential therapeutic strategies for pleomorphic adenomas.