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Thermal response of synchronous CHO cells with different shapes
N N Smith1, W F Harvey, J S Bedford
1Department of Radiation Oncology and Nuclear Medicine, Jefferson Cancer Institute, Thomas Jefferson University, Philadelphia, PA 19107.
Abstract:
This study examines the differential heat sensitivity of rounded suspension cells versus flattened monolayer cells. G1 populations of two different Chinese hamster ovary lines were used to eliminate possible cell cycle artifacts. The cell populations were heated at 43 and 45 degrees C. In all cases, cells treated in suspension were less sensitive to heat killing than cells treated as monolayers.