Ultrastructure of the macaque ciliary ganglion

The primate ciliary ganglion is an obligatory relay in the pathways that control the lens and pupil for the near response and the light reflex, two functions which have been the target of increasing inquiry in behavioural physiology paradigms. This investigation provides a comprehensive description of the ultrastructure of the ciliary ganglion in the rhesus monkey (Macaca mulatta). The results indicate that the ciliary ganglion contains a heterogeneous population of neurons in terms of somatic size, cytoplasmic contents and somatodendritic distribution of terminals. Variations in the clear and dense-cored vesicle content of the synaptic profiles present in the ganglion suggest that the synaptic inputs are also heterogeneous and may mediate separate functions. Several characteristic ultrastructural features of the macaque ciliary ganglion are noteworthy. Despite the large size of the neuronal somata, most cells do not exhibit contacts directly onto the somatic membrane. However, the few somata that do receive direct input often display several axosomatic contacts. The vast majority of synaptic interactions occur in the perisomatic neuropil, where the postsynaptic elements consist of simple and complex somatic appendages, as well as dendrites with their appendages. There is little neuropil independent of these immediately perisomatic regions. In some cases, axonal terminals form the central element of complex glomeruli, in which they are presynaptic to numerous spine-like profiles. In other cases, axon terminals and their postsynaptic targets are found within shallow depressions in the somatic membrane or, occasionally, deeply embedded within the borders of the postganglionic neuron. The somata and all the non-myelinated neuronal elements are surrounded by interdigitating, electron-dense processes of satellite cells. These glial cells are sometimes found in shallow recesses, or deeply embedded within the borders of the neuronal somata. The complexity of the ultrastructure of the ciliary ganglion in the macaque suggests that this ganglion may not be a simple relay in the parasympathetic outflow to the eye, but may instead be the site of neuronal processing of the preganglionic input.