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[Cell morphology and surface markers in three patients with granular lymphocyte proliferative disorders of the T-cell
We studied the relationship between cell morphology and surface markers in three patients with granular lymphocyte proliferative disorders. In the two patients with CD3+ CD4-CD8+ cells, there were no atypical cells, and the granules were uniformly fine: In the third patient, who had CD3+ CD4+ CD8- cells, atypical nuclei and the irregular granules in in size were observed. The granules were stained with beta-glucuronidase showing localized coarse features and made clumps, in the staining pattern characteristic of granular lymphocytes. The patient with CD4- CD8+ cells showed cytotoxic T cell phenotype, and the other DN cells type (usually associated with a CD3+ CD4- CD8- phenotype). We obtained conflicting data about OK-NK and Leu 11 antibodies, which recognized CD16 antigen.
We studied the relationship between cell morphology and surface markers in three patients with granular lymphocyte proliferative disorders. In the two patients with CD3+ CD4-CD8+ cells, there were no atypical cells, and the granules were uniformly fine: In the third patient, who had CD3+ CD4+ CD8- cells, atypical nuclei and the irregular granules in in size were observed. The granules were stained with beta-glucuronidase showing localized coarse features and made clumps, in the staining pattern characteristic of granular lymphocytes. The patient with CD4- CD8+ cells showed cytotoxic T cell phenotype, and the other DN cells type (usually associated with a CD3+ CD4- CD8- phenotype). We obtained conflicting data about OK-NK and Leu 11 antibodies, which recognized CD16 antigen.