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Mayaro virus proteins

J M Mezencio1, M A Rebello

  • 1Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brasil.

Memorias Do Instituto Oswaldo Cruz
|April 1, 1993
PubMed
Summary

Mayaro virus structural proteins were identified and characterized. The 34 KDa protein was the first Mayaro virus protein synthesized in infected cells.

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Area of Science:

  • Virology
  • Molecular Biology
  • Cell Biology

Background:

  • Mayaro virus is an arbovirus of the Alphavirus genus, responsible for Mayaro fever.
  • Understanding the structural proteins of Mayaro virus is crucial for developing antiviral strategies.

Purpose of the Study:

  • To characterize the structural proteins of Mayaro virus.
  • To identify viral protein synthesis kinetics in infected cells.

Main Methods:

  • Mayaro virus propagation in BHK-21 cells.
  • Purification of Mayaro virus using potassium tartrate density gradient centrifugation.
  • Electron microscopy for viral particle size determination.
  • Identification and molecular weight determination of viral proteins using SDS-PAGE (implied).

Main Results:

  • Purified Mayaro virus particles are enveloped, with an average diameter of 69 +/- 2.3 nm.
  • Three major structural proteins (p1, p2, p3) with molecular weights of 54 KDa, 50 KDa, and 34 KDa were identified.
  • Six viral proteins were detected in infected cells, including structural proteins and three precursor processing products (62 KDa, 64 KDa, 110 KDa).
  • The 34 KDa protein (p3) was the earliest detected viral protein, synthesized at 5 hours post-infection in both BHK-21 and Aedes albopictus cells.

Conclusions:

  • The study elucidates the structural protein composition of Mayaro virus.
  • The 34 KDa protein is identified as an early-synthesized viral component, potentially significant for viral replication.
  • These findings contribute to the fundamental understanding of Mayaro virus biology.

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