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Verotoxin-binding in human renal sections

C A Lingwood1

  • 1Department of Microbiology, Hospital for Sick Children, Toronto, Ont., Canada.

Nephron
|January 1, 1994
PubMed
Summary

Verotoxin-producing Escherichia coli (VTEC) infections can cause hemolytic uremic syndrome (HUS) in children. This study found VTEC toxin binds to infant glomeruli, suggesting a risk factor for HUS development.

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Area of Science:

  • Nephrology
  • Microbiology
  • Pediatrics

Background:

  • Hemolytic uremic syndrome (HUS) is a leading cause of acute renal failure in children.
  • Gastrointestinal infections with verotoxin-producing Escherichia coli (VTEC) are strongly linked to HUS.
  • The verotoxin (VT) receptor in the kidney is crucial for understanding HUS pathogenesis.

Purpose of the Study:

  • To investigate the binding sites of VT1 in human renal tissue.
  • To compare VT binding in infant and adult kidneys.
  • To confirm the specific glycolipid receptor for VT in renal tissue.

Main Methods:

  • Incubation of frozen human renal sections (infant biopsies and adult autopsies) with fluorescein-conjugated VT1.
  • Microscopic examination of VT binding patterns.
  • Pretreatment of sections with alpha-galactosidase to confirm receptor specificity.

Main Results:

  • VT1 primarily bound to distal convoluted tubules and collecting ducts in both infant and adult kidneys.
  • Significant VT1 binding was observed in infant glomeruli, but not in adult glomeruli.
  • Alpha-galactosidase pretreatment abolished VT1 binding, confirming globotriaosyl ceramide as the specific receptor.

Conclusions:

  • The globotriaosyl ceramide glycolipid is the specific receptor for VT.
  • Differential localization of this glycolipid in the pediatric renal glomerulus may be a risk factor for HUS development after VTEC infection.

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