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BCG cell imaging using scanning probe microscopy

A A Garcia1, W C Pettigrew, J Graham

  • 1Department of Chemical, Biological, and Materials Engineering, Arizona State University, Tempe 85287-6006.

Scanning Microscopy
|June 1, 1993
PubMed
Summary
This summary is machine-generated.

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Scanning tunneling microscopy (STM) and atomic force microscopy (AFM) visualized intact bacille Calmette Guerin (BCG) cells, revealing surface details and extracellular microgranules. STM offered higher resolution, detailing periodic layers and corroborating electron microscopy findings.

Area of Science:

  • Microbiology
  • Surface Science
  • Nanotechnology

Background:

  • Mycobacterium tuberculosis, including bacille Calmette Guerin (BCG) strains, presents unique surface characteristics.
  • Understanding BCG cell surface morphology is crucial for diagnostics and therapeutic development.

Purpose of the Study:

  • To image whole, intact BCG cells using Scanning Tunneling Microscopy (STM) and Atomic Force Microscopy (AFM).
  • To compare the capabilities of STM and AFM in visualizing BCG surface topography and extracellular features.
  • To correlate AFM and STM findings with established microscopy techniques.

Main Methods:

  • BCG cells were immobilized on glass slides (AFM) or highly oriented pyrolytic graphite (STM).
  • AFM imaging involved centrifugal deposition, fixation, and dehydration.

Related Experiment Videos

  • STM imaging was performed in aqueous solution, leveraging BCG hydrophobicity for adhesion.

    • Main Results:

    • AFM revealed BCG cells in characteristic cord arrangements and identified extracellular microgranules not visible with light microscopy.
    • STM provided higher resolution surface details, including periodic layers, consistent with transmission electron microscopy.
    • Both techniques demonstrated the feasibility of imaging intact BCG cells, with STM offering superior feature discrimination.

    Conclusions:

    • AFM and STM are effective tools for high-resolution imaging of intact BCG cells.
    • STM provides greater surface detail and corroborates findings from electron microscopy.
    • These techniques enhance the understanding of BCG cell surface architecture and extracellular components.