Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Current X-ray diffraction experiments using a synchrotron radiation source

N Yagi1, S Takemori, M Watanabe

  • 1Department of Pharmacology, Tohoku University School of Medicine.

Advances in Experimental Medicine and Biology
|January 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diversion of the sign of phototaxis in a Chlamydomonas reinhardtii mutant incorporated with retinal and its analogs.

FEBS letters·1992
Same author

Site-directed mutation makes rabbit calcyclin dimer.

FEBS letters·1992
Same author

Subfrontal schwannoma--case report.

Neurologia medico-chirurgica·1992
Same author

[Neurotoxicity of antiepileptic drugs].

No to shinkei = Brain and nerve·1992
Same author

Effect of multiple irradiation with low doses of gamma-rays on morphological transformation and growth ability of human embryo cells in vitro.

International journal of radiation biology·1992
Same author

Leiomyosarcoma of the esophagus: report of a case and preoperative evaluation by CT scan, endoscopic ultrasonography and angiography.

Gastroenterologia Japonica·1992
Same journal

Peptidomics in the Spotlight: Advanced Sample Treatment Techniques and Analytical Insights.

Advances in experimental medicine and biology·2026
Same journal

Methods for the Investigation of Protein-Ligands Interactions.

Advances in experimental medicine and biology·2026
Same journal

Sample Preparation Strategies for Microbial Cell Surface Proteomics: Integrating Shaving and Shotgun Approaches.

Advances in experimental medicine and biology·2026
Same journal

Proteomic Sample Preparation for the Petroleum Industry: A Biocorrosion Case Study.

Advances in experimental medicine and biology·2026
Same journal

Proteomic and Functional Comparison of Extracellular Vesicles from Wild-Type and Lyn-Deficient Stromal Cells.

Advances in experimental medicine and biology·2026
Same journal

Proteomic Analysis of Histone Sequence Variants and Post-translationally Modified Forms.

Advances in experimental medicine and biology·2026
See all related articles

New X-ray diffraction techniques using synchrotron radiation and Fuji imaging plates enable detailed study of muscle fibers. These advancements reveal distinct myosin head arrangements during active muscle shortening versus isometric contraction.

Area of Science:

  • Biophysics
  • Structural Biology
  • Muscle Physiology

Background:

  • X-ray diffraction is crucial for studying biological materials.
  • Recent innovations include Fuji imaging plates and synchrotron radiation, advancing X-ray diffraction experiments.
  • Skeletal muscle structure and function are key areas of biological research.

Purpose of the Study:

  • To present results from recent X-ray diffraction experiments on skeletal muscle fibers.
  • To demonstrate the utility of new imaging technologies for muscle research.
  • To investigate structural changes in muscle during contraction and shortening.

Main Methods:

  • Utilized synchrotron radiation at Photon Factory, Tsukuba.
  • Employed Fuji imaging plates for high-resolution, 2D X-ray diffraction pattern recording.

Related Experiment Videos

  • Performed experiments on single skinned fibers and whole frog muscles.
  • Implemented time-resolved experiments with an imaging-plate exchanger and mechanical shutter.

    • Main Results:

    • Recorded 2D diffraction patterns from single rabbit/frog fibers with 30-sec exposure, showing layer-lines up to 5.1 nm.
    • Observed Ca2+-activated fiber patterns similar to electrically stimulated live muscle.
    • Time-resolved patterns from maximally shortening frog muscle showed weakened actin layer-lines (5.1, 5.9 nm) and specific myosin reflections (14.3, 7.2 nm).
    • Noted intensification of the second meridional reflection from the thick filament during shortening.

    Conclusions:

    • Single fiber X-ray diffraction simplifies structural and biochemical experiments.
    • Time-resolved diffraction reveals distinct myosin head arrangements during active shortening compared to isometric contraction.
    • These findings provide insights into the molecular mechanisms of muscle contraction.