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Phase-modulation fluorescence lifetime immunoassay of dichlorprop
F Garcia Sanchez1, A Navas, J Lovillo
1Departamento de Química Analítica, Facultad de Ciencias, Universidad de Málaga, Spain.
Analytical Biochemistry
|November 1, 1993
Summary
This study introduces a novel immunofluorimetric method for detecting dichlorprop herbicide. The technique accurately quantifies free and antibody-bound dichlorprop simultaneously using fluorescence lifetime differences.
Area of Science:
- Analytical Chemistry
- Environmental Science
- Biochemistry
Background:
- Dichlorprop herbicide detection is crucial for environmental monitoring.
- Existing methods may lack simultaneous quantification of free and bound forms.
- Fluorescence-based techniques offer potential for sensitive immunoassays.
Purpose of the Study:
- To develop a homogeneous immunofluorimetric assay for dichlorprop.
- To enable simultaneous determination of free and antibody-bound dichlorprop.
- To validate the assay's analytical performance.
Main Methods:
- Utilized phase-resolved and modulation-resolved fluorescence measurements.
- Employed fluoresceinamine-labeled dichlorprop.
- Analyzed fluorescence lifetimes and phase-angles for quantification.
- Performed multifrequency heterogeneity analysis.
Main Results:
- Distinguished free and bound dichlorprop by fluorescence lifetime differences (600 ps).
- Achieved a mean relative standard deviation of 6% for calibration curves.
- Established a practical dynamic range of 0.1 to 100 µg/mL.
- Demonstrated simultaneous quantification of both species was feasible.
Conclusions:
- The developed homogeneous immunofluorimetric technique allows precise, simultaneous quantification of dichlorprop.
- Fluorescence lifetime analysis provides a reliable basis for herbicide detection.
- The method exhibits favorable analytical specifications for practical applications.