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Related Experiment Videos

Human leukemic intermediate DNA components

G Corneo, E Ginelli, E Polli

    Acta Haematologica
    |January 1, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Human leukemic DNA fractionation reveals two intermediate DNA components on a methylated albumin kieselguhr (MAK) column. These components differ in molecular weight and elution profiles, suggesting distinct genomic distributions.

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    Area of Science:

    • Molecular Biology
    • Genomics
    • Biochemistry

    Background:

    • DNA fractionation is crucial for understanding genome organization.
    • Human leukemic leucocytes provide a model for studying DNA heterogeneity.
    • Methylated albumin kieselguhr (MAK) chromatography separates DNA based on structural properties.

    Purpose of the Study:

    • To investigate the distribution of intermediate DNA components on a MAK column.
    • To characterize the properties of different intermediate DNA fractions.
    • To explore the genomic location of distinct DNA components.

    Main Methods:

    • DNA extraction from human leukemic leucocytes.
    • Fractionation of DNA using methylated albumin kieselguhr (MAK) column chromatography.

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  • Reannealing of DNA fractions to a Cot value of 20 mol·sec/L.
  • Analysis of DNA density using cesium chloride (CsCl) gradients.

    • Main Results:

    • Two intermediate DNA components were identified with distinct densities (1.703 g/ml and 1.707 g/ml).
    • High molecular weight intermediate DNA (1.703 g/ml) eluted early with the main DNA peak.
    • Low molecular weight intermediate DNA (1.707 g/ml) showed broader elution, enriched in later fractions.

    Conclusions:

    • Intermediate DNA in human leukemic cells comprises at least two distinct components.
    • These components exhibit differential elution from MAK columns, correlating with molecular weight and density.
    • The findings suggest differential genomic organization and potential interspersion of low molecular weight DNA within homogeneous genomic regions.