Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mouse genes encoding DNA topoisomerase I

B Baumgärtner1, C Klett, H Hameister

  • 1Division of Biology, Universität Konstanz, FRG.

Mammalian Genome : Official Journal of the International Mammalian Genome Society
|January 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sequential targeting of CFTR by BAC vectors generates a novel pig model of cystic fibrosis.

Journal of molecular medicine (Berlin, Germany)·2011
Same author

Retigabine, a K(V)7 (KCNQ) potassium channel opener, attenuates L-DOPA-induced dyskinesias in 6-OHDA-lesioned rats.

Neuropharmacology·2011
Same author

[Transforaminal lumbar interbody fusion for the treatment of degenerative spondylolisthesis].

Der Orthopade·2011
Same author

Complete electric dipole response and the neutron skin in 208Pb.

Physical review letters·2011
Same author

Double-slit experiments with microwave billiards.

Physical review. E, Statistical, nonlinear, and soft matter physics·2011
Same author

Exceptional points in a microwave billiard with time-reversal invariance violation.

Physical review letters·2011

Researchers analyzed the mouse type I DNA topoisomerase gene (Top-1) and its pseudogene (Top-1ps). They mapped Top-1 to chromosome 2 and Top-1ps to chromosome 16, revealing evolutionary insights into these important genetic elements.

Area of Science:

  • Genetics
  • Molecular Biology
  • Evolutionary Biology

Background:

  • Type I DNA topoisomerase (Top-1) is a crucial enzyme for DNA replication and transcription.
  • Understanding the genetic structure and evolution of Top-1 and its related pseudogenes provides insights into genome stability and evolution.
  • Previous studies have characterized the human TOP1 gene and its pseudogene.

Purpose of the Study:

  • To genetically analyze the mouse type I DNA topoisomerase (Top-1) gene.
  • To determine the exon-intron structure of the mouse Top-1 gene.
  • To map the active mouse Top-1 gene and identify any related pseudogenes in the mouse genome.

Main Methods:

  • Gene sequencing and structural analysis to determine exon-intron boundaries.
  • Chromosomal mapping techniques to locate the active gene and pseudogene.

Related Experiment Videos

  • Comparative analysis of mouse and human gene sequences and structures.

    • Main Results:

    • The exon-intron structure of the mouse Top-1 gene is highly similar to the human TOP1 gene.
    • The active mouse Top-1 gene was mapped to distal Chromosome 2.
    • A truncated, processed topoisomerase-I-related pseudogene (Top-1ps) was identified on mouse Chromosome 16.
    • The Top-1ps locus on mouse Chr 16 and the human TOP1P2 pseudogene on human Chr 22 define a conserved linkage group.
    • Shared structural features between mouse and human pseudogenes suggest ancient origins predating mammalian radiation.

    Conclusions:

    • The mouse type I DNA topoisomerase gene (Top-1) shares significant structural similarity with its human counterpart.
    • The mapping of Top-1 to mouse Chr 2 and the pseudogene Top-1ps to mouse Chr 16 provides a genetic framework for further studies.
    • The conserved linkage group involving the Top-1ps pseudogene suggests its ancient evolutionary origin, established before the divergence of mammalian species.