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Identification of complex t(15;17) in APL by FISH

Z Chen1, R Morgan, J F Stone

  • 1Cancer Center of Southwest Biomedical Research Institute, Scottsdale, Arizona.

Cancer Genetics and Cytogenetics
|January 1, 1994
PubMed
Summary

Fluorescence in situ hybridization (FISH) effectively identifies the RAR-alpha/PML fusion in acute promyelocytic leukemia (APL). This study details FISH

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FISH - Fluorescent In-situ Hybridization02:07

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...