Related Experiment Videos
Detection of ampicillin resistance in Enterococcus spp. by disk diffusion and two commercial automated systems
B M Willey1, B N Kreiswirth, G Williams
1Princess Margaret Hospital, University of Toronto, Ontario, Canada.
Abstract:
A total of 154 Enterococcus faecium and 48 Enterococcus faecalis isolates, 55% of which were ampicillin-resistant, were used to evaluate two commonly used automated systems, Vitek's GPS-TA card (bioMérieux, USA) and MicroScan's POS MIC Type 6 panel (Baxter Health-care, USA), and the NCCLS disk diffusion method for the detection of ampicillin resistance. MicroScan panels were read by the Walk/Away system and by visual inspection. The results were as follows: disk diffusion, 100% sensitive and specific; Vitek, 100% sensitive and 95% specific; and MicroScan, when read by the Walk/Away, 85% sensitive and 98% specific. When MicroScan was interpreted by visual inspection, however, sensitivity increased to 96%.