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Cisplatin blocks mechano-electric transducer current in chick cochlear hair cells
T Kimitsuki1, T Nakagawa, K Hisashi
1Department of Otorhinolaryngology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Hearing Research
|December 1, 1993
Abstract:
The effects of cisplatin (cis-dichlorodiammine platinum II, CDDP) on the mechano-electrical transduction (MET) current were investigated with a whole-cell patch-electrode voltage clamp technique in dissociated cochlear hair cells of chicks. CDDP blocked the MET channel in a dose- and voltage-dependent manner. At -50 mV, CDDP blocked the MET channel with a Hill coefficient of approximately 2 and a dissociation constant (KD) of 1.5 x 10(-3) M. The kinetics of CDDP blockade consist of a voltage-independent and a voltage-dependent component.