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Affinity purification of histidine-tagged proteins
J Schmitt1, H Hess, H G Stunnenberg
1European Molecular Biology Laboratory, Gene Expression Programme, Heidelberg, Germany.
Molecular Biology Reports
|October 1, 1993
Abstract:
Expression of recombinant proteins is a standard technique in molecular biology and a wide variety of prokaryotic as well as eukaryotic expression systems are currently in use. A limiting step is often the purification of the expressed recombinant protein, particularly if mammalian expression systems that yield low expression levels are employed. Here, we discuss the advantages and restrictions of tagging recombinant proteins with histidines and purifying them by Ni(2+)-NTA chromatography.