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Updated: Aug 11, 2026

Proteomic Profiling of Macrophages by 2D Electrophoresis
Published on: November 4, 2014
Identification of A1 macroglobulin and A2 macroglobulin in rat serum by a two-dimensional quantitative
Abstract:
A1 macroglobulin was identified in rat serum by a two-dimensional quantitative immunoelectrophoresis with intermediate gel containing monovalent anti-A1M rat serum. A second serum macroglobulin A2M antigenically related to A1M was identified in turpentine-stimulated or irradiated rats.
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Western Blotting
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
Two-dimensional Gel Electrophoresis
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