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Perfusion imaging of the rat kidney with MR
D S Williams1, W Zhang, A P Koretsky
1Pittsburgh NMR Center for Biomedical Research, Carnegie Mellon University, Pittsburgh, PA 15213.
Purpose:
To develop a technique for measurement of regional renal perfusion with magnetic resonance (MR) imaging.
Materials And Methods:
Quantitative renal perfusion images in rats were obtained by measurement of the reduction in kidney MR image signal intensity after steady state magnetic labeling of arterial blood in the suprarenal aorta. Labeling was achieved with adiabatic fast passage inversion of arterial water.
Results:
Cortical renal blood flow was 4.9 mL/g/min +/- 0.15 (12 rats), which correlated well with previous measurements obtained with other techniques. Serial perfusion images obtained every 5 minutes during intravenous infusion of either acetylcholine or angiotensin II showed that one increased and the other decreased renal blood flow, respectively, also correlating with previous measurements.
Conclusion:
Quantitative measurement of cortical renal blood flow can be obtained with proton MR imaging techniques, with use of endogenous arterial water as a tracer. This technique should be readily applicable to measurement of renal perfusion in humans.
Insights
This study presents a new magnetic resonance (MR) imaging technique for measuring renal perfusion. The method accurately quantifies cortical renal blood flow in rats using arterial water as a tracer.
Area of Science:
- Biomedical Imaging
- Renal Physiology
- Magnetic Resonance Imaging
Background:
- Accurate measurement of renal perfusion is crucial for diagnosing and managing kidney diseases.
- Existing techniques for assessing renal blood flow have limitations.
- Magnetic resonance (MR) imaging offers potential for non-invasive perfusion assessment.
Purpose of the Study:
- To develop and validate a novel MR imaging technique for quantitative measurement of regional renal perfusion.
- To assess the feasibility of using endogenous arterial water as a tracer for renal perfusion.
- To establish a reliable method for monitoring changes in renal blood flow.
Main Methods:
- Developed a quantitative MR imaging technique utilizing steady-state magnetic labeling of arterial blood in the suprarenal aorta.
- Employed adiabatic fast passage inversion of arterial water for magnetic labeling.
- Acquired serial perfusion images in rats at 5-minute intervals.
Main Results:
- Achieved accurate quantitative measurement of cortical renal blood flow (4.9 mL/g/min +/- 0.15) in rats, correlating well with previous methods.
- Demonstrated the technique's ability to detect changes in renal blood flow induced by acetylcholine and angiotensin II infusion.
- Validated the use of endogenous arterial water as an MR imaging tracer for renal perfusion.
Conclusions:
- Proton MR imaging techniques can quantitatively measure cortical renal blood flow using endogenous arterial water.
- The developed MR imaging technique provides a reliable and non-invasive method for assessing renal perfusion.
- This technique holds significant promise for clinical application in human renal perfusion studies.